Submission Guidelines

 

Complete a Sample Submission Form and print a copy to accompany your samples. We can only accept samples if we receive a hardcopy of your submission form together with them. If you are submitting more than 12 samples please submit sample names in an Excel spreadsheet. Email the Excel form to genomicscore@vcu.edu

 

DNA Submission Requirements

  • Submitted DNA should be free of contaminants and high molecular weight. When DNA QA/QC results are atypical, we will require your approval before continuing with the sample. We cannot guarantee results with samples of dubious quality.
  • The concentrations listed below should be determined by fluorometry (e.g. Picogreen assay, Qubit, etc.). If you do not have access to a fluorometry-based assay and are relying on a spectrophotometer (e.g., the Nanodrop), submit at least five times what is requested. Nanodrop tends to over-estimate DNA/RNA concentration.
  • DNA samples should be provided in EB or TLE buffer. Note: The most critical requirement is that buffers contain no more than 0.1 mm EDTA.
  • The 260/280 ratio should be 1.7 - 2.0 and the 260/230 ratio should be ~2.0.

RNA Submission Requirements

  • RNA should be free of contaminants and substantially intact, with a BioAnalyzer RIN number of 7 or higher. When an RNA profile is atypical, we require your approval before continuing with the sample. We cannot guarantee results with samples of dubious quality.
  • The concentrations listed below should be determined by Fluorometry (e.g. Picogreen assay, Qubit, etc.) or Agilent BioAnalyzer. If you do not have access to a fluorometry-based assay and are relying on spectrophotometry such as Nanodrop, provide at least three times what is requested.
  • RNA should be provided in Molecular Biology Grade Water.
  • The 260/280 ratio should be 1.7 - 2.0.

Instructions for naming your samples

  • Submit samples in 1.5 ml tubes, labeled clearly with submission date and sample identifier.
  • Name samples with a 4 letter and 4 digit number identifier. For example: George Maggiano is gema0001.
  • If you have submitted samples to the Genomics Core in the past, continue using sequentially numbered samples.