Illumina Systems

 

HiSeq 4000

Illumina Hiseq 4000 instrument 

Read Length: 1 x 50, 2 x 75, 2 x 150 b

№ of lanes per flow cell: 8

№ of reads per lane: 300 M reads

Avg. output per lane (2 x 75 b): 45 Gb

Avg. output per lane (2 x 150 b): 90 Gb

Applications:

- RNA-seq

- Metagenomic/transcriptomic

- Exome-seq

- Human targeted

- Human genome

- Other

NextSeq 500

Illumina NextSeq 500 instrument 

Read Length Available:

  • 1 x 75 b
  • 2 x 75 b
  • 2 x 150 b

№ of lanes per flow cell: 1

Mid Output:

  • № of reads per run: 130 M reads
  • Avg. output per run (2 x 150 b): 40 Gb

High Output:

  • № of reads per run: 400 M reads
  • Avg. output per lane (2 x 150 b): 120 Gb

Applications:

- RNA-seq

- Metagenomic/ transcriptomic

- Exome-seq

- Human targeted

- Human genome

- Bacterial genome

- Other

MiSeq

Illumina MiSeq instrument 

Read Length available:

  • 1 x 75 b
  • 2 x 75 b
  • 2 x 150 b
  • 2 x 300 b

№ of lanes per flow cell: 1

№ of reads per run: 25 M reads

Avg. output per run (2 x 300 b): 15 Gb

Applications:

- 16S rRNA microbiome profile

- Amplicon-seq

- De novo bacterial sequencing

- RNA-seq

- Human targeted

- Other