The HydroShear is used for fragmenting DNA to sizes larger than 1KB. HydroShear offers the simplest, most reproducible, and most controllable method available for generating random DNA fragments. This device uses hydrodynamic shearing forces to fragment DNA strands. HydroShear can handle any source of DNA at any concentration in volumes as small as 40μl. DNA fragments can be sheared within a two-fold size distribution (e.g. if the target size is 2kb, the HydroShear can be calibrated to produce a distribution centered on 2kb with 90% of the DNA falling between 1.3kb and 2.6kb. It is the best system for making long DNA fragments for single molecule sequencing such as PacBio Sequel.